Sequence Length statistics issue

Hello everyone,

I am running the 13 paired sequence (16S) data.

I got the quality reads as follow:
demux-paired-end.qzv (289.2 KB)

I trimmed the primer using cutadapt and then I using DADA2 for trimming and truncating. I used the command as

I got the following:
stats-dada2-trimmed.qzv (1.2 MB)

After feature table construction, I got the following,
rep-seqs-trimmed.qzv (296.1 KB)

I am wondering If i truncated the sequences at 200, why I am getting minimum length as 249 and maximum length as 312 in the sequence length statistics table. I guess both values should be 200 (As it was same truncating value in moving picture tutorial i.e 120).

Is the merging not proper?

Please help! Thank you so much in advance!

A post was merged into an existing topic: How to determine whether fastq files are already demultiplexed?