Hello Everyone,
I tried importing the output generated from split_library in qiime1 using the command:
qiime tools import
–input-path seqs.fna
–output-path seqs.qza
–type SampleData[Sequences]
I got a message that it is not a Demuxformat file? Pls. let me know how I can solve this. Thank you very much.
Hello Nicholas,
I tried putting the source format QIIME1DemuxFormat, it still came back with a message that seqs.fna is not a QIIME1DemuxFormat file, is there another way to import this file to qiime2?
Thanks,
Raya
Hi @Pseudomonas84,
Thanks for attempting to specify the source format. Could you please run the following command (on the unimported data) and share the output: head seqs.fna tail seqs.fna
I want to inspect the first/last several lines to make sure this is not a formatting issue.
It is a bit of a shot in the dark — but I can tell you that lowercase nucleotide characters can cause issues with some plugins downstream.
Another possibility is if there are duplicate sequence IDs. That should not occur, but might if you did anything unusual with the qiime1 outputs prior to trying to import (e.g., concatenating multiple seqs.fna files?). I can’t really give support on qiime1 commands, but it could help to give some context on the qiime1 commands that you used upstream to generate these files.
Hello Nicholas,
The above suggestion worked. Thank you very much.
I went back to moving pictures tutorial to try to do taxonomy barplot, so I had to use the feature classifier first, I got a plugin error:
qiime feature-classifier classify-sklearn \
--i-classifier gg-13-8-99-515-806-nb-classifier.qza
--i-reads rep-seqs.qza
--o-classification taxonomy.qza
Plugin error from feature-classifier
Hi Matthew,
Thanks for the reply. I made this input and got the error message below.
qiime feature-classifier classify-sklearn --i-classifier gg-13-8-99-515-806-nb-classifier.qza --i-reads rep-seqs.qza --o-classification taxonomy.qza
Plugin error from feature-classifier:
Debug info has been saved to /tmp/qiime2-q2cli-err-r8vn3hyh.log