Hello Everyone, I tried importing the output generated from split_library in qiime1 using the command: qiime tools import --input-path seqs.fna --output-path seqs.qza --type SampleData[Sequences] I got a message that it is not a Demuxformat file? Pls. let me know how I can solve this. Thank…

Hi @Pseudomonas84 , I think the issue is that you are missing the following parameter from your command: --source-format QIIME1DemuxFormat Could you give that a try? Thanks!

Hello Nicholas, I tried putting the source format QIIME1DemuxFormat, it still came back with a message that seqs.fna is not a QIIME1DemuxFormat file, is there another way to import this file to qiime2? Thanks, Raya

Hi @Pseudomonas84 , Thanks for attempting to specify the source format. Could you please run the following command (on the unimported data) and share the output: head seqs.fna tail seqs.fna I want to inspect the first/last several lines to make sure this is not a formatting issue. Thanks!

heads seqs.fna KDC_1 D00420 org_bc=GAGATGAC new_bc=GAGATGAC bc_diffs=0 TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCgcgcgTAGGTGGTTTGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAAAACTGACTGACTAGAGTGTGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACCAACACTGACACTGA…

hm... those certainly look like the correct format but I do notice a bunch of lowercase characters, which might be causing this import issue. what version of QIIME2 are you using? Could you try the following? cat seqs.fna | tr 'acgt' 'ACGT' > new_seqs.fna qiime tools import \ –input-path new…

Hello Nicholas, The above suggestion worked. Thank you very much. I went back to moving pictures tutorial to try to do taxonomy barplot, so I had to use the feature classifier first, I got a plugin error: qiime feature-classifier classify-sklearn \ --i-classifier gg-13-8-99-515-806-nb-classifie…

Hi @Pseudomonas84 , can you please copy and paste the full error message output when you run the command with the --verbose flag?

Hi Matthew, Thanks for the reply. I made this input and got the error message below. qiime feature-classifier classify-sklearn --i-classifier gg-13-8-99-515-806-nb-classifier.qza --i-reads rep-seqs.qza --o-classification taxonomy.qza Plugin error from feature-classifier: Debug info has bee…

Hi @Pseudomonas84 , can you please copy and paste the full error message output when you run the command with the --verbose flag? For example: $ qiime feature-classifier classify-sklearn \ --i-classifier gg-13-8-99-515-806-nb-classifier.qza \ --i-reads rep-seqs.qza \ --o-classification taxonom…

Hello Matthew, My VM crashed and I had to restart it and input the command again, and it worked, no errors...weird. Thanks a lot!

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Seqs.fna is not a(n) QIIME1DemuxFormat file

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