Running Kraken2/moshpit - error 71 help please

I've been following the tutorial at q2-books/q2-shotgun/q2-shotgun/ at main · caporaso-lab/q2-books · GitHub to classify my sequences using kraken and the PlusPF database. Creating the indices [Importing Kraken2 and bracken databases into qiime2!] seemed to work fine, but I can't classify.

When I try to run
qiime moshpit classify-kraken2 --i-seqs paired-end-demux.qza --i-kraken2-db k2_ppf.qza --p-threads 4 --output-dir kraken_ppf --p-report-minimizer-data
I get the error message:
Plugin error from moshpit: An error was encountered while running Kraken 2, (return code 71), please inspect stdout and stderr to learn more. Debug info has been saved to /tmp/qiime2-q2cli-err-kdmkgpta.log
I can't find stdout, stderr or a log file anywhere in my system, so does anyone know what this error could mean?

Thanks for your help!

1 Like

Hello @karenhn,

Could you run the command again but additionally with the --p-verbose flag and post the output?

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