Run fragment-insertion without dada2 - failed

[Robin_Mjelle]

I am trying to run fragment-insertion without first running dada2 (because it takes so long) but get the following error:

qiime tools import
--type "SampleData[SequencesWithQuality]"
--input-format SingleEndFastqManifestPhred33V2
--input-path ./manifest.tsv
--output-path ./demux_seqs.qza

qiime fragment-insertion sepp
--i-representative-sequences ./demux_seqs.qza
--i-reference-database sepp-refs-gg-13-8.qza
--o-tree ./tree.qza
--o-placements ./tree_placements.qza
--p-threads 5

                There was a problem with the command:                     

(1/1) Invalid value for "--i-representative-sequences": Expected an artifact
of at least type FeatureData[Sequence]. An artifact of type
SampleData[SequencesWithQuality] was provided.

[thermokarst]

Hey there @Robin_Mjelle!

@Robin_Mjelle

I am trying to run fragment-insertion without first running dada2

Well, that explains the error message, doesn't it?

@Robin_Mjelle

Invalid value for “–i-representative-sequences”: Expected an artifact
of at least type FeatureData[Sequence]. An artifact of type
SampleData[SequencesWithQuality] was provided.

The error is indicating that you're providing the wrong type of data as input.

Backing up, have you tried increasing the number of threads when running DADA2?