Run fragment-insertion without dada2 - failed

Technical Support
1

I am trying to run fragment-insertion without first running dada2 (because it takes so long) but get the following error:

qiime tools import
–type “SampleData[SequencesWithQuality]”
–input-format SingleEndFastqManifestPhred33V2
–input-path ./manifest.tsv
–output-path ./demux_seqs.qza

qiime fragment-insertion sepp
–i-representative-sequences ./demux_seqs.qza
–i-reference-database sepp-refs-gg-13-8.qza
–o-tree ./tree.qza
–o-placements ./tree_placements.qza
–p-threads 5

                There was a problem with the command:

(1/1) Invalid value for “–i-representative-sequences”: Expected an artifact
of at least type FeatureData[Sequence]. An artifact of type
SampleData[SequencesWithQuality] was provided.

3

Hey there @Robin_Mjelle!

Well, that explains the error message, doesn't it?

The error is indicating that you're providing the wrong type of data as input.

Backing up, have you tried increasing the number of threads when running DADA2?