My colleagues and I used the qiime2 picrust2 plugin, and we are working on analyzing the results. We are now interested in reverse mapping back to which taxa/sequences are associated with the different KO, EC, and pathways we are interested in. Does anyone know if this is possible?
It is possible with the standalone version, but not with the QIIME 2 plugin currently. You can see how to get stratified outputs (i.e. links between specific ASVs/OTUs to functions that they contribute) in this tutorial: PICRUSt2 Tutorial (v2.3.0 beta) · picrust/picrust2 Wiki · GitHub.