Regarding errors with denoise in MiSeq runs

[ChrisKeefe]

Welcome to the forum, @Pnmrc!

I did a quick search on this forum for your error:

@Pnmrc

Filtering Error in names(answer) <- names1 :
‘names’ attribute [4] must be the same length as the vector [3]

I suspect it's a cryptic out-of-memory error, caused by running this command with many cores.

Denoising error, names attribute must be the same length as vector

Hi @Roger_Huerlimann, Congratulations for running into one of the more cryptic error messages that dada2 can throw at you. This is effectively a memory error even though it does not look like one — or more specifically a multithreading issue that can be fixed by reducing the number of threads used. See here for some discussion: https://forum.qiime2.org/t/dada2-error-return-code-1/7140?u=nicholas_bokulich I think the dada2 version was upgraded somewhere in there, so it could be a version issu…

Try re-running with fewer cores, and let us know how it goes!

Best,
Chris