Reference database (97%) for qiime vsearch cluster-features-closed-reference

Hi @PatoUru,

You will need to make it yourself! We supply a set of files that we pre-processed with RESCRIPt, including the SILVA 138 99% reference files on the Q2 data resources page, which you can use as a starting point.

This tutorial describes how we prepared those files, as well as how you could prepare the 97% OTUs. You should be able to just re-cluster the 99% OTUs to generate a 97% OTUs file, see the command in this section:

4 Likes