Reads joining questions

Hi, I cheched the Atacama tutorial because I’m working with Paired-end reads and I have some questions:
I have imported fastq files containing the forwards and reverse sequences with the following comand: qiime tools import
–type ‘SampleData[PairedEndSequencesWithQuality]’
–input-path pe-64-manifest
–output-path paired-end-demux.qza
–input-format PairedEndFastqManifestPhred33

Next Do I need to Join the reads following the comand from the tutorial “Alternative methods of read-joining in QIIME 2”?
Or Do I follow the “Atacama” tutorial that don’t ferform the joing of the reads?
Thank you for your kind help

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1 Like

Hi!
If you will follow the protocol one of the next steps will be denoising with DADA2. At this step your reads will be joined, so if you don’t have other aims you can just proceed with the protocol. Another good and detailed tutorial is “Moving Pictures”

3 Likes

Ok, I’ll try that, thank you!

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