I am pretty new to QIIME2 but i went through the tutorials successfully. I have some 22 QIIME1 paired end fastq files that I wanted to re-analyze using QIIME2.
First stumbling block is that the paired end reads seem to already have been demultiplexed as each set of paired ends correspond to specific samples. How do I undo that step using QIIME2 if possible? and have one pair of paired end reads to represent all samples for QIIME2. I no longer have QIIME1 on my system but I can always re-install it if I need to use it.
Next problem, how do I create the barcodes.fastq.gz file?
Is there any particular reason you want to re-multiplex your reads? Personally, if my data was already demuxed, I would want to import and start working from there!
We have some very relevant documentation for you here in the Importing Tutorial - please take a look and lets us know how it goes!
Thanks for responding. I will check out that tutorial.
I wanted to re-multiplex because all my samples are in separate fastq files and I wanted to get them back to one set of paired end fastq files to be used in QIIME2. And I wasn’t sure I could import already demultiplexed files or I may have not remembered :o
I am still not sure what to do about my missing barcodes.fastq.gz file…
