Thank you for your time. After reading all posts related to the rarefaction curve posts in the forum. Some questions still confusing for me.
I got a rarefaction curve in QIIME2, which is so confusing. Based on my understanding, the curve converges to the flat curve is the symbol of enough sequencing depth.
But in the plot of QIIME2 rarefaction curve. No matter how few reads the sample has, the curve will reach converge.
In my sample, J1 and F1 are exactly the same DNA, which I sequenced twice, while F3/J3, F5/J5 are in the same fashion. From QIIME2 produced curve I really do not know how to distinguish from them, which is better.
Enclosed is the qzv file I analyzed. May I have some suggestions on how to diagnose the sequencing depth is enough or not from the QIIME2 produced curve?? From my current result, which sample is enough sequenced?
Appreciate your help!!! Tons of thanks!
alpha-rarefaction.qzv (636.5 KB)