Questions about QIIME 2 Metadata

[drmusk (Muhammad Umar)]

Dear Colleagues
Thanks for helping me so far. It means a lot. I successfully imported 55 sequences using manifest file.
Since now I am running qiime dada2 denoise-paired which may take I think several hours for 55 samples on personal laptop.
I am worried how can I run next analysis where I will be needing metadata file for groups comparison. I want to ask 2 things here.

1. After denoising I need to run “qiime feature-table summarize” which requires metadata file. How can I develop this command
2. In subsequent analysis, I need grouping for statistical analysis. Like I have these sequences from 4 geographical locations. How can I compare alpha and beta diversity among these groups.

Once again thank you for this all

[thermokarst]

Hi @drmusk! This was just discussed today in the following thread:

Difference between metadata and manifest file

I am getting confused between manifest and matadata file? I understand that menifest file contain information about sample ID , path to fastq files and orientation of reads. It generally used to import the fastq files in Qiime2. In Qiime1, we generally used mapping file so it contaion information about sample id, linkers, barcode, description. It is mention that in Qiime 2 we call this file as matadata file. Where do we exactly use the matadata file? I do not have barcode and linker then shoul…

Please take a look at the entire thread and let us know if you have any questions.