Thanks for helping me so far. It means a lot. I successfully imported 55 sequences using manifest file.
Since now I am running qiime dada2 denoise-paired which may take I think several hours for 55 samples on personal laptop.
I am worried how can I run next analysis where I will be needing metadata file for groups comparison. I want to ask 2 things here.
After denoising I need to run “qiime feature-table summarize” which requires metadata file. How can I develop this command
In subsequent analysis, I need grouping for statistical analysis. Like I have these sequences from 4 geographical locations. How can I compare alpha and beta diversity among these groups.