Hello Qiime2 team,
I have a question about the summary output of the longitudinal linear-mixed-effects plugin. Below is the command that I entered and attached are the metadata.tsv and the first-distances.qza files. I am using qiime2019.1 (conda environ in Ubuntu 18).
qiime longitudinal linear-mixed-effects --m-metadata-file metadata.tsv --m-metadata-file weighted-firstdist.qza --p-state-column StudyDayN --p-individual-id-column pig --p-formula “Distance ~ StudyDayN*StudyGroup” --o-visualization weighted-lme.qzv --p-metric Distance
I am trying to analyze the first distances with StudyDayN and StudyGroup as fixed effects. The pig column is the id of the sample, StudyDayN is the longitudinal factor, StudyGroup is another fixed factor. After running, the weighted-lme.qzv is successfully created, but the summary section of the results do not look correct. Specifically, the No. Groups and min/max/mean samples per group are not what I expected. Based on the attached metadata.tsv, I expected 10 groups with a min of 5 per group, max of 6 per group and mean of >5 per group. But the results summary has No. Groups as 27 and min/max as 1/2, respectively. Is my formula or individual-id not identified correctly?