Quality plot after importing data

Hello Experts,

Please see the attached qzv file that I got after running the qiime demux summarize command. The quality plot doesn't run smooth and has discrete bars. Please check if there is some wrong with the sequences that I got from the sequencing facility.

The command that i ran

qiime tools import
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path ccmtbac
--input-format CasavaOneEightSingleLanePerSampleDirFmt
--output-path ccmt-demux-paired-end.qza

qiime demux summarize
--i-data ccmt-demux-paired-end.qza
--o-visualization ccmt-demux-paired-end.qzv

ccmt-demux-paired-end.qzv (313.5 KB)


Hi @gsshankar, welcome to :qiime2:!

It appears that these may have been pre-processed in some way. Are you sure these are the raw data files? This appears quite similar to what is discussed in this thread:


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