Qiime2Picrust_Relative abundance of KO values

Hi Everyone,

I am working in Qiime-2-Picrust.

I want to determine the relative abundance of KO values to find which genes/enzymes /proteins are different among groups. How could it be correlated with microbiome data?

And also how can I determine the gene category level 1, 2, or 3?

I have ko_metagenome.qzv, ec_metagenome.qzv, rarefied_table.qzv, table.qzv, feature-table.tsv and other related files (eg., jaccard emperor, bray curtis emperor) at hand.

Do you have any idea about how to proceed with these analyses?

Thanks in advance.

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