I have had a wonderful time learning about QIIME2 so far. This forum has been very informative. I thank the community members for their continued help and support.
I had the following questions that I would love to hear people's thoughts on:
- In the FeatureTable, if a sample shows a decreased frequency of features (or OTUs), does that mean the sample integrity has been compromised or sample may have been degraded during the initial sequencing steps?
- As I performed the steps of the "moving pictures" tutorial, I learned that there was only the Greengenes database that was used for the taxonomic profiling. Is there a way to choose a database of out liking while carrying out the QIIME pipeline?
I look forward to hearing from the community.
Thank you in advance,