qiime2 dada2 setting

[Yiggrite (Yiggrite Wu)]

Hi,

My demux.qza is like this:

how should I set the number in qiime2 dada2 denoise-paired?

i used these numbers from the tutorial, but i got 6 samples with feature count 0.
qiime dada2 denoise-paired **

--i-demultiplexed-seqs paired-end-demux.qza **

--p-trim-left-f 13 **

--p-trim-left-r 13 **

--p-trunc-len-f 150 **

--p-trunc-len-r 150 **

--o-table table.qza **

Is this the problem?
Thanks

[cherman2 (Chloe Herman)]

Hi @Yiggrite
We are gonna need more info to be able to help you!

1. What does your interactive quality plot look like?

2. What region are you working with?

3. What sequencing length did you order 2 x 150, 2 x 250 something else?

4. What tutorial are you using?

Have you checked out our qiime2 youtube channel? This channel has videos that can help answer your question. https://www.youtube.com/@QIIME2