qiime tools import error: forward and reverse reads do not match

Thank you very much,I had solve the problem.
but there is another problem.
when try this code
qiime tools import
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path FASTQ_files_untrimmed/Sequencing_run_2_FASTQ
--input-format CasavaOneEightSingleLanePerSampleDirFmt
--output-path Import/Untrimmed_files/import_descriptive_mycobiome_article_sequencing_run_2.qza

I have the following error:
There was a problem importing FASTQ_files_untrimmed/Sequencing_run_2_FASTQ:
FASTQ_files_untrimmed/Sequencing_run_2_FASTQ is not a(n) CasavaOneEightSingleLanePerSampleDirFmt:
These samples do not have matching pairs of forward and reverse reads: {'Sample77562', 'Sample58363'}
what happened?

Hi @fdoctor,

It looks like there are two samples that dont have a forward and reverse read.
If you go into the FASTQ_files_untrimmed/Sequencing_run_2_FASTQ directory you should see that these sample names only occur once while the rest will occur twice.

Do you know why there wouldn't be paired fastq files for those samples?
:turtle:

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Thank you very much. I have solved the problem.

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