Thank you very much,I had solve the problem.
but there is another problem.
when try this code
qiime tools import
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path FASTQ_files_untrimmed/Sequencing_run_2_FASTQ
--input-format CasavaOneEightSingleLanePerSampleDirFmt
--output-path Import/Untrimmed_files/import_descriptive_mycobiome_article_sequencing_run_2.qza
I have the following error:
There was a problem importing FASTQ_files_untrimmed/Sequencing_run_2_FASTQ:
FASTQ_files_untrimmed/Sequencing_run_2_FASTQ is not a(n) CasavaOneEightSingleLanePerSampleDirFmt:
These samples do not have matching pairs of forward and reverse reads: {'Sample77562', 'Sample58363'}
what happened?