qiime fragment-insertion sepp

嗨!大家好。我在做帕金森小鼠教程时,输入qiime fragment-insertion sepp
--i-representative-sequences ./dada2_rep_set.qza
--i-reference-database sepp-refs-gg-13-8.qza
--o-tree ./tree.qza
--o-placements ./tree_placements.qza
--p-threads 1
进行系统发育树的建立,但是却报错了:

*Plugin error from fragment-insertion:

Command '['run-sepp.sh', '/tmp/qiime2/qiime2/data/45310018-d692-44ed-bbce-446a66c14f5c/data/dna-sequences.fasta', 'q2-fragment-insertion', '-x', '1', '-A', '1000', '-P', '5000', '-a', '/tmp/qiime2/qiime2/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/aligned-dna-sequences.fasta', '-t', '/tmp/qiime2/qiime2/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/tree.nwk', '-r', '/tmp/qiime2/qiime2/data/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt']' returned non-zero exit status 1.*
他没有给我解决方法,所以我向万能的朋友们求助,这种情况应该怎么解决呀?感谢您的帮助。

Hi @HEQI,

Welcome to the :qiime2: forum! :wave:

Can you please re-run your command with the --p-debug flag and copy/paste the output in your reply? This is a special flag for sepp - it will run a bit more slowly, but will produce a much more detailed output log (the log right now basically tells us nothing, its like "hey, i broke"). Thanks! :lizard:

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