I have been trying to rerun demultiplexed samples obtained from our sequencing center. I have gotten to the point that I want to visualize my dada2 output, but am receiving an error with my metadata file. I ran a check on keemei and received an error because the sample ids provided had “_” instead of “-” (Example: Lisa001_0023-8-5-2015). I tried converting that character, and still have the same problem. I found a similar topic in the forum and made sure that my file was saved in UTF-8 format, but that didn’t help. Any insight would be helpful!
There was an issue with loading the file Mapping_file_012820.csv as metadata:
Found unrecognized ID column name ‘\ufeffsample-id,barcode-sequence,Diet’ while searching for header. The first column name in the header defines the ID column, and must be one of these values:
Case-insensitive: ‘feature id’, ‘feature-id’, ‘featureid’, ‘id’, ‘sample id’, ‘sample-id’, ‘sampleid’
Case-sensitive: ‘#OTU ID’, ‘#OTUID’, ‘#Sample ID’, ‘#SampleID’, ‘sample_name’
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
Find details on QIIME 2 metadata requirements here: https://docs.qiime2.org/2018.11/tutorials/metadata/
Mapping_file_012820.csv (3.2 KB)