qiime feature-classifier extract-reads settings and average running time?


I am trying to build a classifier using “silva_132_99” database. Here is the command I am using:

qiime feature-classifier extract-reads --i-sequences /media/sf_470Proj/99_otus.qza --p-f-primer GTGCCAGCMGCCGCGGTAA --p-r-primer GGACTACHVHHHTWTCTAAT --p-trunc-len 211 --o-reads /media/sf_470Proj/10-rep-seqs-v4.qza

The above command has been running for 24hrs+ but there’s no output. I getting worry that if I had done something wrong in the settings? My partner and I are wondering on whether or not we should use the --p-trunc-len option, and if we should, then how can we determine the length that we want to truncate. It’s also is our main question.

Also, since this is our first experience with qiime2, we would like to know how long the command would take in average (assuming the settings are correct).

I installed QIIME2 with Oracle Virtual Box. Any help would be greatly appreciated.

Kiet H.

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