qiime demux emp-paired A duplicate barcode was detected

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1

Hey!
I am running qiime2 version 2023.2.0, and I want to use the qiime demux emp-paired command.
however when I run it I get the error message:

 A duplicate barcode was detected. The barcode ACTTAGACTCTT was observed for samples 10317.000076578 and 10317.000074464.

what alternative options do I have?
Thank you
Nadav

2

Hi @nadavlisha,

You can only have one barcode per sample. So, check to make sure you do not have any accidentally repeated barcodes.

If multiple runs were used to generate the data... that is, multiple samples across different runs... Then it'd not be unusual for different samples on different runs to have the same barcode... Thus, you'll have to import these separately.

Ideally, you'd denoise with DADA2 on a per-run basis. Then merge the resulting table and sequence files prior to downstream analyses.

3

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