Hi, does the silva-138-99-seqs.qza database contain species-level taxonomic information? Is it possible to use the headers in a custom fasta file as a taxonomy.tsv file, and if so, what are the parsing rules? Thanks.
Yes — as with other QIIME 2 artifacts, you can check out the provenance to confirm the processing steps, and since this artifact was generated with RESCRIPt, the SILVA taxonomy parsing steps are recorded in provenance:
I’m not 100% sure what you mean, but yes in theory you can include any kind of annotation information, including sequence IDs, in a taxonomy file.
The taxonomy files used by QIIME 2 consist of TSVs in this format:
So you can make a custom database of any kind, so long as it conforms to that format.