Hello, I have some old MiSeq 16S rRNA PE sequencing data. They are from old project (at that time, we only have QIIME 1). The data are are fasta format. They are from three batches of sequencing.
Here the details that how I generated the data.
After I received raw data (fastq file) from sequencing center, I do some basic QC for fastq files. Then I run join_paired_ends.py firstly and then split_libraries_fastq.py. In the end, I got a fasta file. I combine three demulplex fasta file and three mapping file. I get a total fasta file + combined mapping file. From here, I can easily build OTU table using whatever methods such as “pick_closed_reference_otus.py” in QIIME1.
I am now switching to QIIME2 and I would like to see how taxonomy looks using the QIIME2 methods.
I read QIIME 2 tutorials. Almost all tutorials start with raw fastq data from sequencing center. However, it’s been long time. The center didn’t save the raw files (fastq files). I only have a fasta file and a mapping file.
Is there anyway that I can use these two files in QIIME2. Simple conversion etc.