I´m trying to perform the differential abundance analysis with gneiss but I can´t figure out the problem.
qiime gneiss add-pseudocount
–i-table table.qza
–p-pseudocount 1
–o-composition-table composition.qza
It says Error: QIIME 2 plugin ‘gneiss’ has no action ‘add-pseudocount’.
This command was removed one year ago, and was replaced with inline-parameters for controlling the psuedocount value. Are you following an old tutorial somewhere? What version of QIIME 2 are you using?
yes I´m following the old tutorial from here: https://docs.qiime2.org/2018.2/tutorials/gneiss/
I´m using qiime 2, 2019.1 . So since the tutorial doesn’t work, can someone put up the new code or new tutorial??
Just a note of interest: the tutorials/documentation will always work because they are automatically run and tested as a part of every single release... this keeps us from accidentally releasing broken documentation! (some details of the text may get stale if we fail to catch it, but the code will always run fine since it is automatically tested)