I’m using q2-picrust2’s
custom-tree-pipeline as described in the q2-picrust2 tutorial, and the default
max-nsti cutoff described there is
2. I noticed that the Hidden state prediction page says “sequences with extremely high NSTI values (e.g. > 1) should be removed”, and further down on that page: “There is no clear cut-off for a high NSTI values, but a good rule of thumb is that sequences placed with NSTI > 0.15 will be less reliable.”
Is the default
max-nsti=2 reasonable for human fecal samples, or is it better to use something more stringent as described on the HSP wiki page, such as
Thanks for your insight!