Pyrosequence work in Qiime2 (Barcode)

[You_y_Choi (Y__Y)]

Many thanks @colinbrislawn !

In my understanding,

1. use 'convert_fastaqual_fastq.py' to create a FASTQ file.
2. Then, import it using 'SingleEndFastqManifestPhred33V2'.
3. Next, utilize Cutadapt to remove the selected primer information.
4. Afterwards, proceed with 'dada2-denoise-single' for denoising.

Is that correct?

Thank you for your support !