Hey there @turtleonaleash!
The problem here is that the sample IDs need to be the same in both bray_curtis_pcoa_results_initial.qza
and bray_curtis_pcoa_results_final.qza
in order for this viz to work. One way to do that is to relabel the sample ids in your filtered feature tables.
# Example QIIME 2 Sample Metadata
sample-id time pairingid
a initial sample1
b final sample1
c initial sample2
d final sample2
e initial sample3
f final sample3
# create "initial" feature table
qiime feature-table filter-samples \
--i-table table.qza \
--m-metadata-file md.tsv \
--p-where "time='initial'" \
--o-filtered-table initial-table.qza
# this file should have samples a, c, e
# create "final" feature table
qiime feature-table filter-samples \
--i-table table.qza \
--m-metadata-file md.tsv \
--p-where "time='final'" \
--o-filtered-table final-table.qza
# this file should have samples b, d, f
# relabel sample ids in initial table
qiime feature-table group \
--i-table initial-table.qza \
--p-axis sample \
--m-metadata-file md.tsv \
--m-metadata-column pairingid \
--p-mode sum \
--o-grouped-table initial-table-relabeled.qza
# this file should have samples sample1, sample2, sample3 (corresponding to a, c, e)
# relabel sample ids in final table
qiime feature-table group \
--i-table final-table.qza \
--p-axis sample \
--m-metadata-file md.tsv \
--m-metadata-column pairingid \
--p-mode sum \
--o-grouped-table final-table-relabeled.qza
# this file should have samples sample1, sample2, sample3 (corresponding to b, d, f)
Now, use the newly relabeled tables to run your core-metrics (x2) (don't forget to choose the same even sampling depth). Also, you will need to create a new metadata file for this step, one with the new "pairingid" as your main "id" column.
Keep us posted! :qiime2: