Problems with Importing Data 10/24

User Support
2

Hi Adam,

You will need to do this:

  1. You need a manifest file which will be a file with columns" Sample-id, forward-absolute-path, reverse-absolute-path similar to what's here: Importing data — QIIME 2 2019.7.0 documentation

  2. The files unfortunately will have to be copied into a folder. You can try this mv command here: command line - How Can I Merge Multiple Directories into One - Ask Ubuntu

  3. Then your command needs to be replaced to: --input-path needs to be /Users/adam/Desktop/Sunflower.../manifest.txt

  4. The absolute file paths will be /Users/Adam/desktop/folder/forward.fastq1.fastq.gz, /Users/Adam/desktop/folder/reverse.fastq1.fastq.gz, etc. (and should be in the same ROW, not column)

  5. You can see someone with this same issue here: importing paired FASTQ file into qiime had errors. any help? - #42 by ben

Ben

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