The reason is that you should use "SingleEndFastqManifestPhred33V2" instead of "SingleLanePerSamplePairedEndFastqDirFmt" when importing with a manifest file.
Please, double check that you want to import it as single end reads, cause in your ḿanifest file there are both F and R reads. For paired reads, use "PairedEndFastqManifestPhred64V2".
Thanks for the reply! I had tried PairedEndFastqManifestPhred64V2 earlier, and got this error:
**An unexpected error has occurred:**
**No transformation from <class 'q2_types.per_sample_sequences._format.PairedEndFastqManifestPhred64V2'> to <class 'q2_types.per_sample_sequences._format.SingleLanePerSampleSingleEndFastqDirFmt'>**
**See above for debug info.**
That's why I tried using SingleLanePerSamplePairedEndFastqDirFmt.
Thanks for the suggestion, but I get a similar error to when I try the Phred64V2 offset.
Traceback (most recent call last):
File "/programs/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/builtin/tools.py", line 157, in import_data
artifact = qiime2.sdk.Artifact.import_data(type, input_path,
File "/programs/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/sdk/result.py", line 240, in import_data
return cls._from_view(type_, view, view_type, provenance_capture,
File "/programs/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/sdk/result.py", line 265, in _from_view
transformation = from_type.make_transformation(to_type,
File "/programs/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/core/transform.py", line 58, in make_transformation
raise Exception("No transformation from %r to %r" %
Exception: No transformation from <class 'q2_types.per_sample_sequences._format.PairedEndFastqManifestPhred33V2'> to <class 'q2_types.per_sample_sequences._format.SingleLanePerSampleSingleEndFastqDirFmt'>
An unexpected error has occurred:
No transformation from <class 'q2_types.per_sample_sequences._format.PairedEndFastqManifestPhred33V2'> to <class 'q2_types.per_sample_sequences._format.SingleLanePerSampleSingleEndFastqDirFmt'>
See above for debug info.