Possible Analysis Pipeline for Ion Torrent 16S Metagenomics Kit Data in QIIME2?

Hey @Nicholas_Bokulich and @colinbrislawn

I have a follow up question about my vsearch taxonomy classifier. One of my regions has a higher percentage of unclassified taxa than the other and I'm thinking I could potentially tweak some of the vsearch settings in the "qiime feature-classifier classify-consensus-vsearch" command.

I am currently using the default parameters (as I have in my code in my response to @Lauren

I am curious if you have any suggestions for parameters to adjust as a starting point to try and improve this unclassified tax %?

My DADA2 stats in this region are pretty good and nothing changed when I increased the truncation length of the segment pulled from greengenes so I think tweaking the vsearch parameters would be a good next testing point. :thinking:

Appreciate any thoughts/suggestions you may have, thank you!
Katherine

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