Hey @Nicholas_Bokulich and @colinbrislawn
I have a follow up question about my vsearch taxonomy classifier. One of my regions has a higher percentage of unclassified taxa than the other and I'm thinking I could potentially tweak some of the vsearch settings in the "qiime feature-classifier classify-consensus-vsearch" command.
I am currently using the default parameters (as I have in my code in my response to @Lauren
I am curious if you have any suggestions for parameters to adjust as a starting point to try and improve this unclassified tax %?
My DADA2 stats in this region are pretty good and nothing changed when I increased the truncation length of the segment pulled from greengenes so I think tweaking the vsearch parameters would be a good next testing point.
Appreciate any thoughts/suggestions you may have, thank you!
Katherine