Plugin error in longitudinal pairwise differences

Dear User Support,

I am using longitudinal pairwise differences and I used the following code. I am getting a plugin error as given below. My metadata file contains information for state second for group CNr. I am not understanding why does it return this error.

qiime longitudinal pairwise-differences --m-metadata-file Sample_metadata_long_exp.tsv --m-metadata-file core-metrics-results_long_exp/shannon_vector.qza \

--p-metric shannon_entropy
--p-group-column staging
--p-state-column time
--p-state-1 First
--p-state-2 Second
--p-individual-id-column unique-id
--p-replicate-handling random
--o-visualization pairwise-differences_long_exp.qzv

Plugin error from longitudinal:

State Second is not represented by any members of CNr group in metadata. Consider using a different group_column or state value.

Debug info has been saved to /tmp/qiime2-q2cli-err-x1_a0gqq.log

It would be great to get an answer for this problem.

Thank you


That means that there are no state "Second" in column "time", check metadata.


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