Plugin error from Taxa:Feature ids missing

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1

dear qiime forum,

I was trouble with taxa barplot where I typing as :

(qiime2-2019.10) haqqy@LAPTOP-3OTV2K3C:/mnt/e/GUT CONTENT EPINEPHELUS/training_classifier$ qiime taxa barplot --i-table GUT_CONTENT_COI130_table.qza --i-taxonomy GUT_CONTENT_COI_taxonomy.qza --m-metadata-file Metadata_COI_Rajaampat.tsv --o-visualization GUT_CONTENT_COI-taxa-bar-plots.qzv
and trace back like this
Plugin error from taxa:

** Feature IDs found in the table are missing from the taxonomy: {'69797f5a6feb800412c4b410b3325799', 'df7a2baabdcbb02420fb1cdf9aade5ec', 'bd4825795694288185de251e95619d33', '6e08e7960b6ca4159d0a11eb1afc645f', '576404272d808a7390cd6b2e36fce7b8', 'a607812f557abc6492e8975f0a8ec1e4', '4ae58eaa7d05353d2a33031b6ca8825a', '5931acd40a41714a0531cc30737a1009', '3887a8089cc4122662b816fc415632e8', '4384c8258036064eb0154000da744d24', '85071f318d11298590c4ea3acf3cbf34', '52f1bcafc8a4362c1c39196965a9125b', '8e39c5a0fbbb000231e831567bf5916c', 'ae5b7ceed1784fc395acea0664dfc650', 'a4600094fc523b1728a7feda80464e37', '4a9bce06adff46ffdbf1bd7549b3e50c', '083d4f15c06413dc0d252dd75ef41607', '9260dc626fba87b77685dffde4e738f1', '651ede8723b2a012efacec8e712f0b65', 'd0a1cfa394497f408f3a58413e85553f', '58401b650095a746f615ec33b2c638ae', '029a79736e0aff1ccd78b0fbfb9d7d11', 'd9b576f0614eeb2b17eaeb61ecd18aa4', 'fb00d7ad0c056b7278da8280e4b551de', '0c5cee815a5fb0a68f7bf5b4e45d1c76'}**

Debug info has been saved to /tmp/qiime2-q2cli-err-2aiusztc.log
(qiime2-2019.10) haqqy@LAPTOP-3OTV2K3C:/mnt/e/GUT CONTENT EPINEPHELUS/training_classifier$ qiime taxa barplot --i-table GUT_CONTENT_COI130_table.qza --i-taxonomy GUT_CONTENT_COI_taxonomy.qza --m-metadata-file Metadata_COI_Rajaampat.tsv --o-visualization GUT_CONTENT_COI-taxa-bar-plots.qzv

would you help me how to solving this trouble?thank you

2

Hi! Did you filter your table before taxonomy assignment? Looks like you filtered it first, then classified and now providing unfiltered table and taxonomy for already filtered table

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3

i was trying filter with command:

(qiime2-2019.10) haqqy@LAPTOP-3OTV2K3C:/mnt/e/GUT CONTENT EPINEPHELUS/training_classifier$ qiime taxa barplot --i-table id-filtered-table.qza --i-taxonomy GUT_CONTENT_COI_taxonomy.qza --m-metadata-file Metadata_COI_Rajaampat.tsv --o-visualization GUT_CONTENT_COI-taxa-bar-plots.qzv
Saved Visualization to: GUT_CONTENT_COI-taxa-bar-plots.qzv

but why any sample was removed from taxa and unexpected by first sample like thisGUT_CONTENT_COI130-stats.qzv (1.2 MB) GUT_CONTENT-filtered-table.qzv (1.2 MB)

4

Hi @haqqy.26,
Check out the provenance on your files... the issue is that you are mixing up files, using a feature table and sequences from different runs of dada2 with different truncation settings! Thus, the feature IDs in your table and sequences (and taxonomy) do not match. Make sure you are using the correct files, you can use the provenance as a guide.

Good luck!

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5

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