I run into the problem while creating a box plot after I feature grouped my table.
This is what I do:
qiime feature-table group --i-table FLORA_combo_table.qza \
–p-axis sample \
–m-metadata-file FLORA_combo_mm.txt \
–m-metadata-column ‘Run’ \
This works perfectly, I get my .qza file, then follows this:
This is where the problem is - you cannot use the same sample metadata file again, because the feature-table group command has effectively built new sample ids:
You will need to manually construct a new sample metadata file with these new sample ids. It is very likely that most of the old sample metadata won’t make sense now (because you might have multiple column values collapsed down into a single “sample”).
I group by 'Run', where J33PD and others are 4 names for 4 runs.
I cannot just simply use those 4 names instead of #Sample ID..
Im sorry but Im so confused here.
Is there any way to know the 'correct' sample name to get the box plots?
Hi @Kotryna, nothing has changed here - sample metadata has always been required for this visualization. As I mentioned earlier, you are performing a step (feature-table group) that creates all-new samples, which requires all new sample metadata. Hope that helps!