Hi,
I got an error when I was trying to Trimming ITS samples:
qiime itsxpress trim-pair-output-unmerged
–i-per-sample-sequences demux_trimmed.qza
–p-region ITS1
–p-taxa F
–p-threads 16
–o-trimmed itsxpress_trimmed.qza
–verbose
Plugin error from itsxpress:
/export2/home/yluo/temp/q2-CasavaOneEightSingleLanePerSampleDirFmt-91msip6l/LUO.65_ATGCCGTA_L001_R1_001.fastq.gz is not a(n) FastqGzFormat file:
Missing sequence for record beginning on line 13
I uninstall the conda version of ITSxpress, and install again,then rerun. It gave the same error.
The thing is that it ran successfully for my first batch of ITS samples, however it failed for me second batch of ITS samples, though the same script was used.
Does someone have any suggestions?
Thanks in advance!
Best regards,Yujia