Hello. I am trying to do alpha and beta diversity analyses with AGP (American Gut Project) data and am getting the error shown below the second chunk of code shown below. data.qza is a feature table which is deblurred sequences downloaded from redbiom.
Any help would be greatly appreciated! Thank you
qiime phylogeny align-to-tree-mafft-fasttree \
--i-sequences data_rep_seqs.qza
--p-parttree
--o-alignment aligned-rep-seqs.qza
--o-masked-alignment masked-aligned-rep-seqs.qza
--o-tree unrooted-tree.qza
--o-rooted-tree rooted-tree.qza
qiime diversity core-metrics-phylogenetic \
--i-phylogeny mafft-fastree-out2/rooted_tree.qza
--i-table data.qza
--p-sampling-depth 100
--m-metadata-file AGP-metadata.tsv
--output-dir core-metrics-results
Plugin error from diversity:
The table does not appear to be completely represented by the phylogeny.