Plugin error from demux: No sequences were mapped to samples


qiime demux emp-paired
--i-seqs emp-paired-end-sequences_run1.qza
--m-barcodes-file run1-metadata.tsv
--m-barcodes-column BarcodeSequence \
--o-per-sample-sequences demux.qza
--o-error-correction-details demux-details.qza

Plugin error from demux:

No sequences were mapped to samples. Check that your barcodes are in the correct orientation (see the rev_comp_barcodes and/or rev_comp_mapping_barcodes options). If barcodes are NOT Golay format set golay_error_correction to False.

Debug info has been saved to /var/folders/h1/7zgcmgzn3r3g30pq3s0k5zzh0000gn/T/qiime2-q2cli-err-3e9p6165.log

Please let me know how can i fix the above error
Thank you


Have you tried using the rev_comp_barcodes or the rev_comp_mapping_barcodes? You might want to unzip your barcodes fastq file and look at a few of the barcodes to confirm their orientation/formatting are what you expect them to be.

It is also worth finding out if the barcodes are Golay. If they are not you will want to set --p-no-golay-error-correction when running your analysis. You may need to ask your sequencing center and/or look through any documentation they provided you with to know if this is the case.


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