Mac OS X (Terminal)
Miniconda
qiime2-2017.10
I’ve been running into an issue while running the following deblur command:
- qiime deblur denoise-16S --i-demultiplexed-seqs /path/demux-filtered.qza --p-trim-length -1 --o-representative-sequences /path/rep-seqs-deblur.qza --o-table /path/table-deblur.qza --o-stats /path/deblur-stats.qza
Input file was generated using the ‘qiime quality-filter q-score --o-filtered-sequences’ command.
The following error was generated:
-
Plugin error from deblur:
-
Command ‘[‘deblur’, ‘workflow’, ‘–seqs-fp’, ‘/var/folders/37/l3v4ty9d2jdbssnc7cp2f4gw0000gn/T/qiime2-archive-t1csnrsj/6593ebd0-1520-477b-99dd-a500642381d1/data’, ‘–output-dir’, ‘/var/folders/37/l3v4ty9d2jdbssnc7cp2f4gw0000gn/T/tmpg5bhd6xa’, ‘–mean-error’, ‘0.005’, ‘–indel-prob’, ‘0.01’, ‘–indel-max’, ‘3’, ‘–trim-length’, ‘-1’, ‘–min-reads’, ‘10’, ‘–min-size’, ‘2’, ‘–jobs-to-start’, ‘1’, ‘-w’]’ returned non-zero exit status 1
I’m fairly certain everything is installed and working properly but could be wrong. I removed my original 2017.10 environment, cleaned conda’s cache, and updated conda before re-running my entire set of code and still encountering into the same issue. Any suggestions?
Thanks for your time.
Matt