Plugin error from dada2

[Nicholas_Bokulich]

Hi @lipengcheng,
Notice this line in your error message:

@lipengcheng

Mismatched forward and reverse sequence files: 91816, 97358.

At least one pair of your sequence files have mismatched sequence counts. See here for a similar issue and solution:

DADA2 Mismatched forward and reverse sequence files(different counts of every sample)

Dear All, I am a new user of QIIME2, before I imported my fastq.files, I trimmed barcodes and primers, demutiplexed my data into R1,R2 files per sample (I used 'choosetag' function of the Galaxy). After the import, demux-summary-3.qzv was generated. [15%20AM] Then I started to denoise with dada2. Here is my code: qiime dada2 denoise-paired --p-trunc-len-f 223 --p-trunc-len-r 223 --i-demultiplexed-seqs half-paired-end-demux-3.qza --o-representative-sequences rep-seqs-3.qza --o-tabl…

If you performed any kind of quality control prior to importing to QIIME 2, that is most likely what is introducing this error. You can follow the steps in that post to count the sequences in each pair of files to discover which file pair is causing this issue.

Good luck!