I was running a small sample of demux paired-end dataset using the denoise-paired, it works. Then I started to run the whole dataset and got the plugin error as below:
(qiime2-2017.2) Jinbings-iMac:qiime2 jinbingbai$ qiime dada2 denoise-paired \
> --i-demultiplexed-seqs demux-paired-end.qza \
> --o-table table.qza \
> --o-representative-sequences rep-seqs.qza \
> --p-trim-left-f 0 \
> --p-trim-left-r 0 \
> --p-trunc-len-f 200 \
> --p-trunc-len-r 200
Plugin error from dada2:
Command '['run_dada_paired.R', '/var/folders/sd/vlrr9d916gg5qj3yzzf3sr
fw0000gn/T/tmppy7c0qis/forward', '/var/folders/sd/vlrr9d916gg5qj3yzzf3
srfw0000gn/T/tmppy7c0qis/reverse', '/var/folders/sd/vlrr9d916gg5qj3yzz
f3srfw0000gn/T/tmppy7c0qis/output.tsv.biom',
'/var/folders/sd/vlrr9d916gg5qj3yzzf3srfw0000gn/T/tmppy7c0qis/filt_f',
'/var/folders/sd/vlrr9d916gg5qj3yzzf3srfw0000gn/T/tmppy7c0qis/filt_r',
'200', '200', '0', '0', '2.0', '2', '1', '1000000']' returned non-zero
exit status 1
Re-run with --verbose to see debug info.
Could you help me figure out what is wrong?
Thanks, Bing