Hello. I'm hoping for some help with an error during denoising with DADA2. I'm running the following: qiime dada2 denoise-paired --i-demultiplexed-seqs demux.qza --p-trim-left-f 5 --p-trim-left-r 5 --p-trunc-len-f 150 --p-trunc-len-r 150 --o-table table.qza --o-representative-sequen…

What version of QIIME 2 are you running? Can you please activate your QIIME 2 environment and run the following commands: qiime info conda list Thanks!

Yes, of course. Thanks! qiime info produces System versions Python version: 3.5.5 QIIME 2 release: 2018.6 QIIME 2 version: 2018.6.0 q2cli version: 2018.6.0 Installed plugins alignment: 2018.6.0 composition: 2018.6.0 cutadapt: 2018.6.0 dada2: 2018.6.0 deblur: 2018.6.0 demux: 2018.6.0 d…

Did you run both of those commands in the same conda environment? The conda info command seems to indicate that you ran the command in your base environment... [image] Heather_Olins: /gsfs0/public/qiime/miniconda3

I just noticed that the user accounts in this thread swapped --- I thought I was talking to the same person the whole time. In the future @qiuwa , it is usually best to start a new topic, rather than hijack someone else's thread! I split your thread out into a new topic, @qiuwa

Just to clarify, @quiwa and I are working together on this. They are the research computing person, and I'm the biologist. I asked for help answering some of your questions, and then have been monitoring as it seems the issue was related to the installation.

Yes. The same conda enviorment. I installed it and made module to export the path. I install qiime for her group to use. We had the same error. Should I do any variables setting? Thanks, Wei

Thanks @Heather_Olins & @qiuwa ! In the future it is helpful to let us know these kinds of details! :house_with_garden:

Will do. My apologies for the confusion, and thanks very much for all your help with this!

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Plugin error from DADA2 denoise-paired

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