Plugin error from dada2:An error was encountered while running DADA2 in R (return code 1)

[Nicholas_Bokulich]

@huzeyfetas

Calls: removeBimeraDenovo -> isBimeraDenovoTable

This error occurs when none of your reads overlap. See here (and search the forum archive) for more advice on diagnosing and correcting this error:

Plugin error from dada2: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more

Dear All, I am trying to execute DADA2 for quality checking and I got the following error. Ive attached my quality plot and my table data. Is it due to the trimming length? If it is so, what will be appropriate trimming length? I know if i reduce the trimming length, there wont be a sufficient overlap for merging. demux.qzv (287.7 KB) table.qza (287.7 KB) R version 3.4.1 (2017-06-30) Loading required package: Rcpp DADA2 R package version: 1.6.0 Filtering .. Learning Error Rates 2…

@huzeyfetas

system call failed: Cannot allocate memory

You may also not have enough memory. This is probably because your input reads are very noisy (you are not truncating enough), but you can find some more info on this error here:

DADA2 Issue with R

I tried running DADA2 but keeps throwing me the error stated below: Plugin error from dada2: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more. Debug info has been saved to /tmp/qiime2-q2cli-err-e4i52m_r.log I have attached the error log filedebug.txt (2.4 KB) Help would be appreciated