Plugin error fragment-insertion sepp

Hi there,

I have a plugin error using fragment insertion sepp, my command and error message is as following:

qiime fragment-insertion sepp
--i-representative-sequences /mnt/d/16S_WinterData_Files/A_crassa/PairedEndPrimerRemoved/Taxonomy/crassa-sequences-no-mitochondria-no-chloroplast.qza
--i-reference-database  /mnt/d/16S_WinterData_Files/A_crassa/PairedEndPrimerRemoved/Phylogeny/q2-fragment-insertion/sepp-refs-gg-13-8.qza
--o-tree /mnt/d/16S_WinterData_Files/A_crassa/PairedEndPrimerRemoved/Phylogeny/q2-fragment-insertion/crassa-tree.qza
--o-placements /mnt/d/16S_WinterData_Files/A_crassa/PairedEndPrimerRemoved/Phylogeny/q2-fragment-insertion/crassa-tree_placements.qza
--p-threads 4

**Plugin error from fragment-insertion:**

  Command '['run-sepp.sh', '/tmp/qiime2-archive-t5j59hc1/017e3ee0-0a12-478d-afaf-549b7cfe8ed6/data/dna-sequences.fasta', 'q2-fragment-insertion', '-x', '4', '-A', '1000', '-P', '5000', '-a', '/tmp/qiime2-archive-2dgxrgz9/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/aligned-dna-sequences.fasta', '-t', '/tmp/qiime2-archive-2dgxrgz9/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/tree.nwk', '-r', '/tmp/qiime2-archive-2dgxrgz9/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt']' returned non-zero exit status 1.

Debug info has been saved to /tmp/qiime2-q2cli-err-b3ac2iyd.log

I am using qiime2-2022.2.

Can you please guide me how to solve that.

Hi @Sabrin,

Thanks for reaching out!

Can you please re-run your command above with the --verbose and --p-debug flags, and copy/paste the output in your response? This will help us to better identify where the issue is coming from. Thanks! :lizard: