Plugin Error (exit code -9) from q2-dada2

[Cheng50373640 (Cheng)]

hi @thermokarst,

yestoday, i installed qiime2017.9, then I run:
qiime dada2 denoise-single \

--i-demultiplexed-seqs demux.qza
--p-trim-left 5
--p-trunc-len 280
--o-representative-sequences rep-seqs-dada2.qza
--o-table table-dada2.qza

error message:
Plugin error from dada2:

An error was encountered while running DADA2 in R (return code -9),
please inspect stdout and stderr to learn more.

do you know what's going on? thank you in advance

[Cheng50373640 (Cheng)]

I notice I should use qiime data2 denoise-paired,

then i run:
qiime dada2 denoise-paired
–i-demultiplexed-seqs demux.qza
-p-trunc-len-f 280
-p-trunc-len-r 280
–p-trim-left-f 5\
–p-trim-left -r 0
–o-representative-sequences rep-seqs-dada2.qza
–o-table table-dada2.qza

the same error,

then i run:
qiime dada2 denoise-paired
–i-demultiplexed-seqs demux.qza
-p-trunc-len-f 0
-p-trunc-len-r 0
–p-trim-left-f 5\
–p-trim-left -r 0
–o-representative-sequences rep-seqs-dada2.qza
–o-table table-dada2.qza

it looks work, (still running)
so, I don’t know if the parameter i set up was wrong, or something else?

thank you in advance

[ebolyen (Evan Bolyen)]

Hey @Cheng50373640,

Could you provide the log files that are generated from the failures (or re-run with --verbose)? There’s not much we can determine because as the error says, we need to inspect stdout/stderr to learn more. Thanks!