Hi I am trying to demultiplex my file to import it into qiime2.
When I run the cutadapt demux-single command I get the following error.
Plugin error from cutadapt:
Command '['cutadapt', '--front', 'file:/tmp/kumarman/tmpp46nqw0h', '--error-rate', '0.1', '-o', '/tmp/kumarman/q2-CasavaOneEightSingleLanePerSampleDirFmt-q9sjgm9l/{name}.1.fastq.gz', '--untrimmed-output', '/tmp/kumarman/q2-MultiplexedSingleEndBarcodeInSequenceDirFmt-o40b4v2f/forward.fastq.gz', '/tmp/kumarman/qiime2-archive-55_j8nx4/5a538933-b0ef-4110-ae22-5345bf117794/data/forward.fastq.gz']' returned non-zero exit status 1
The command I ran was
qiime cutadapt demux-single --i-seqs Run1_demulti.qza --m-barcodes-file barcodes.tsv --m-barcodes-column Barcodes --o-per-sample-sequences demux_Run1.qza --o-untrimmed-sequences untrim_Run1.qza
Kindly advise.
Cheers,
Manoj