picrust with OTU

Other Bioinformatics Tools
1

I,

I think in the end I am trying to use picrust pipeline.
I am currently following picrust_tutorial
I was just wondering if it is correct to use with OTUs instead of ASVs

I thank you very much

Michela

2

I personally try to use ASVs whenever possible. Clustering sequences runs the risk of combining taxa from different genera or families into one representative sequence, potentially skewing your metagenome predictions. I imagine in some cases this may not be too much of an issue. It really depends on the data.

Also, see my response here.

1 Like
3

Thanks for your kind suggestion,

I am going to set up the ASV pipeline also

Michela