picrust with OTU


I think in the end I am trying to use picrust pipeline.
I am currently following picrust_tutorial
I was just wondering if it is correct to use with OTUs instead of ASVs

I thank you very much


I personally try to use ASVs whenever possible. Clustering sequences runs the risk of combining taxa from different genera or families into one representative sequence, potentially skewing your metagenome predictions. I imagine in some cases this may not be too much of an issue. It really depends on the data.

Also, see my response here.

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Thanks for your kind suggestion,

I am going to set up the ASV pipeline also