Phylogeny result interpretation of ITS sequencing sample


I couldnt interpret anything about my alpha and beta diversity analysis result. Especially in betadiversity analysis it came something like corneal ulcer n=21, n=45.
I gave only 12 sample for analysis, how come it was n=21, n=45. Could anyone help me how to interpret phylogeny result like alpha and beta diversity boxplot and Axis (core-metrics-results)?

Herewith I have attached my result ppt and metadata file for your perusal. Looking forward to your reply.
itssample_metadata.tsv (688 Bytes)

Hi @Asha1,
There are a few different graph types here to consider which are all very different from each other.
First, ordination plots that you show in an emperor plot. PCA or distanced based versions PCoA are dimension reduction methods that are used for exploration mainly (though you can certainly do some hypothesis testing on them later, ex PERMANOVA, adonis etc.). You can read more on these here for example, in short the distance between dots (samples) represents how similar/dissimilar they are, depending on the matrix beind used.
The second plots, alpha diversity boxplots are probably the most straightforward, the 2 boxplots representing IQR of your 2 groups and the corresponding non-parametric krusakl-wallis test that compares the ranks of those groups.
The beta diversity boxplots are a bit trickier to grasp initially and you are certainly not the first person to be thrown off by them. Those n- values do not represent individual samples but rather the total number of distances within samples of a group. Perhaps a bit more thorough explanation of those in this thread can help.
Unfortunately, since one of your groups only has an n=2, this makes statistically sound comparisons difficult and under-powered. I would suggest accepting the results of these tests with a grain of salt and proceed with your interpretation cautiously.

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.