Phred scores too high


I have obtained the Phred scores below which look a bit too high, especially on the forward reads.

Any ideas why this is happening?

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Hi @Parix,
I agree the forward reads look unusually good. But sometimes a good thing is just a good thing and you should be happy!

Unless if you already applied some type of filtering to the data. That would explain the unusually high quality scores. You may want to double-check with the sequencing center just to make sure you have the raw fastq data without any modification.

Enjoy your supremely high-quality data!


My forward reads on V4 16S often look like that, I don't think, at face value there's anything to worry about it. That said:


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