Hi team, Could you please let me know what are the lowest sample size that could be considered acceptable? I did the ordination plot and can see the difference/dissimilarity visually between intestine, feed, and water. The problem is that I have n=2 or n=3 in water/feed that I suspect the PERMANOV…

Hi @MarwaTawfik , My recommendation is to not do analysis with fewer than 5 samples per group. This is kind of the rule of thumb minimum sample size for kruskal wallis. With any permutation test, your space is limited based on the number of samples you have to make combinations. If you have 5 tota…

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PERMANOVA on small sample size?

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