Dear All, I got my raw data generated from QIIME1 pipeline, which the barcode and primer have been trimmed, and low quality sequence has been discarded (I think it is not suitable for DADA2). So after I inputed it to QIIME2, I performed the vsearch cluster-features-de-novo for OTU table and repres…

Hi @Lennon_Lee , I would recommend starting with the closed-reference OTU picking step, rather than closed-reference following de novo clustering. Otherwise you may end up clustering some sequences together in the de novo step which may have hit different reference sequences (and therefore been assig…

I didn't noticed that we have PICRUSt2 plugin!! Thanks for all your advices!!

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Performing close reference clustering for PICRUSt from VSEARCH de novo clustering output?

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