Path doesn't exist

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1

When I put the command:

qiime taxa barplot
--i-table table-deblur.qza
--i-taxonomy taxonomy.qza
--m-metadata-file metadata_3.tsv
--o-visualization taxa-bar-plots.qzv

I get this error:

There was an issue with loading the file metadata_3.tsv as metadata:

Metadata file path doesn't exist, or the path points to something other than a file. Please check that the path exists, has read permissions, and points to a regular file (not a directory): metadata_3.tsv

There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org

Find details on QIIME 2 metadata requirements here: Metadata in QIIME 2 — QIIME 2 2023.2.0 documentation

This error has not happened to me before, what could be happening?

2

Hello @Vanesa_Fernandez,

Are you sure that the file metadata_3.tsv exists in the directory in which you ran that command?

Thanks.

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